Journal: International Journal of Oncology
Article Title: Zinc finger protein-like 1 is a novel neuroendocrine biomarker for prostate cancer
doi: 10.3892/ijo.2023.5486
Figure Lengend Snippet: ZFPL1 expression in the primary PC. (A) The photomicrographs demonstrated the specificity of in situ hybridization. A PC section was treated with sense ZFPL1 siRNA probe (left) or antisense ZFPL1 siRNA probe (right). Only antisense probe hybridized with the PC specimen (right). (Scale bar=100 µ m). (B) The photomicrographs depicted ZFPL1 mRNA expression in prostate sections of different cancer stages in comparison with non-cancer specimens (Scale bar=50 µ m). (C) The representative photomicrographs revealed the presence of ZFPL1-immunopostive cells (red) in a PC prostate section and its matched normal tissue (Scale bar=50 µ m). Nuclear stain is DAPI . The adjacent bar graph presents the mean percentage (n=6) of ZFPL1 immunopositive cells per field (magnification, ×400) in PC vs. matched normal prostate tissue. * P<0.0001 (paired t-test). (D) The photomicrographs on the left showed H&E staining of human PC tissue sample, while those on the right show ZFPL1 (green) and nuclear DAPI (Scale bar=50 µ m). White arrows point to the cancerous areas in all photomicrographs. (E) The representative photomicrographs revealed ZFPL1-immunopsitive cells (Red) and nuclear DAPI in different samples of a US Biomax PC tissue microarray (Scale bar=50 µ m). (F) The bar graph presented the quantitated data of a PC tissue microarray. The mean ± SEM (n=6) IHC index of each specimen in the microarray was calculated and plotted against the stage of PC. The mean IHC index of each cancer group except T1N0MO was significantly different from control. * P<0.005 (One Way ANOVA and Tukey's multiple comparison test). ZFPL1, zinc finger protein like 1; PC, prostate cancer; IHC, immunohistochemistry.
Article Snippet: Frozen primary prostate tumors as well as tissue specimens of BPH and normal prostate were provided by Co-operative Tissue Network (CHTN; Durham, USA).
Techniques: Expressing, In Situ Hybridization, Comparison, Staining, Microarray, Control, Immunohistochemistry